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FABP5 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Fatty acid binding protein 5, epidermal

Gene Aliases

E-FABP; epidermal-type fatty acid-binding protein; epithelial fatty acid-binding protein; Fabpe; fatty acid-binding protein 5; fatty acid-binding protein, epidermal; keratinocyte lipid-binding protein; Kl; Klbp; ma; mal1; PA-FABP; psoriasis-associated fatty acid-binding protein homolog; uncharacterized protein LOC16592.

Gene ID

16592

Accession Number

NP_034764.1

Reactivity

Mouse, Mus musculus

Target

Fatty acid-binding protein 5

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FABP5 ELISA Kit (Mouse) (OKEH03095) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fabp5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Fabp5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fabp5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.083 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

15 kDa

Specificity

Natural and recombinant Mouse Fatty acid-binding protein, epidermal

Protein Length

135

NCBI Gene Symbol

Fabp5

Protein Name

Q05816

Gene Name URL

Fabp5

Nucleotide Accession Number

NM_010634.3

Frequently Asked Questions

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