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S1PR5 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Sphingosine-1-phosphate receptor 5

Gene Aliases

Edg8; Edg-8; endothelial differentiation G-protein-coupled receptor 8; endothelial differentiation, sphingolipid G-protein-coupled receptor, 8; nerve growth factor-regulated G-protein-coupled receptor 1; NRG-1; S1P receptor 5; S1P receptor Edg-8; S1P5; sphingosine 1-phosphate receptor 5; sphingosine 1-phosphate receptor Edg-8.

Gene ID

60399

Accession Number

NP_068543.2

Reactivity

Rat, Rattus norvegicus

Target

Sphingosine 1-phosphate receptor 5

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology S1PR5 ELISA Kit (Rat) (OKEH03090) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S1pr5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for S1pr5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S1pr5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.091 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

42 kDa

Specificity

Natural and recombinant Rat Sphingosine 1-phosphate receptor 5

Protein Length

400

NCBI Gene Symbol

S1pr5

Protein Name

Q9JKM5

Gene Name URL

S1pr5

Nucleotide Accession Number

NM_021775.2

Frequently Asked Questions

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