SERPINH1 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Serine (or cysteine) peptidase inhibitor, clade H, member 1
Gene Aliases
47 kDa heat shock protein; BERF-1; beta enolase repressor factor 1; Cbp; Cbp1; Cbp2; co; collagen binding protein 1; Collagen-binding protein; colligin; gp4; gp46; Hsp; Hsp47; J6; Ser; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2; serine protease inhibitor J6; serpin H1; Serpinh2.
Gene ID
12406
Accession Number
NP_001104513.1
Reactivity
Mouse, Mus musculus
Target
Serpin H1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SERPINH1 ELISA Kit (Mouse) (OKEH03077) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpinh1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Serpinh1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Serpinh1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/ml
Sensitivity
40.1 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
47 kDa
Specificity
Natural and recombinant Mouse Serpin H1
Protein Length
417
NCBI Gene Symbol
Serpinh1
Protein Name
P19324
Gene Name URL
Serpinh1
Nucleotide Accession Number
NM_001111043.1
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