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ANXA5 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Annexin A5

Gene Aliases

Anchorin CII; annexin A5; Annexin V; annexin-5; Anx5; calphobindin I; CBP-I; endonexin II; LC5; lipocortin V; PAP-I; placental anticoagulant protein 4; placental anticoagulant protein I; PP4; thromboplastin inhibitor; VAC-alpha; vascular anticoagulant-alpha.

Gene ID

25673

Accession Number

NP_037264.1

Reactivity

Rat, Rattus norvegicus

Target

Annexin A5

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ANXA5 ELISA Kit (Rat) (OKEH03075) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Anxa5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Anxa5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Anxa5 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

7.8-500ng/mL

Sensitivity

3.9 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

43 kDa

Specificity

Natural and recombinant Rat Annexin A5

Protein Length

405

NCBI Gene Symbol

Anxa5

Protein Name

P14668

Gene Name URL

Anxa5

Nucleotide Accession Number

NM_013132.1

Frequently Asked Questions

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