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PCCB ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Propionyl Coenzyme A carboxylase, beta polypeptide

Gene Aliases

1300012P06Rik; AI314687; PCCase subunit beta; propanoyl-CoA:carbon dioxide ligase subunit beta; propionyl-CoA carboxylase beta chain, mitochondrial; R74805.

Gene ID

66904

Accession Number

NP_001298078.1

Reactivity

Mouse, Mus musculus

Target

Propionyl-CoA carboxylase beta chain, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PCCB ELISA Kit (Mouse) (OKEH03069) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pccb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Pccb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pccb captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%

Sensitivity

0.164 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

58 kDa

Specificity

Natural and recombinant Mouse Propionyl-CoA carboxylase beta chain, mitochondrial

Protein Length

541

NCBI Gene Symbol

Pccb

Protein Name

Q99MN9

Gene Name URL

Pccb

Nucleotide Accession Number

NM_001311149.1

Frequently Asked Questions

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