What is the catalog number for MANBA ELISA Kit (Human)?

The catalog number for MANBA ELISA Kit (Human) is OKEH03056-96W.

What sizes are available for MANBA ELISA Kit (Human)?

MANBA ELISA Kit (Human) is available in the following size(s): 96 Well.

Is MANBA ELISA Kit (Human) in stock?

MANBA ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this product covered by a money-back guarantee?

Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

MANBA ELISA Kit (Human)

Selected Variant

OKEH03056-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Mannosidase beta

Gene Aliases

Beta-mannosidase; beta-mannosidase A; lysosomal beta A mannosidase; MANB1; mannanase; mannase; mannosidase, beta A, lysosomal.

Gene ID

4126

Accession Number

NP_005899.3

Reactivity

Homo sapiens, Human

Target

Beta-mannosidase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MANBA ELISA Kit (Human) (OKEH03056) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human MANBA antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated MANBA and incubated. The MANBA found in the sample or standards competes with the biotinylated MANBA for limited binding sites on the immobilized anti-human MANBA antibody. Excess unbound biotinylated MANBA and sample or standard MANBA is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated MANBA captured in the well and inversely proportional to the amount of MANBA which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.401 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

101 kDa

Specificity

Natural and recombinant Human Beta-mannosidase

Protein Length

879

NCBI Gene Symbol

MANBA

Protein Name

O00462

Gene Name URL

MANBA

Nucleotide Accession Number

NM_005908.3

Price

Frequently Asked Questions

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