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RLN3 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Relaxin 3

Gene Aliases

Insl7; insulin-like 7; insulin-like peptide 7; insulin-like peptide INSL7; prorelaxin R3; relaxin-3.

Gene ID

266997

Accession Number

NP_733767.1

Reactivity

Rat, Rattus norvegicus

Target

Relaxin-3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RLN3 ELISA Kit (Rat) (OKEH03053) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rln3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Rln3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rln3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/mL

Sensitivity

40.3 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

15 kDa

Specificity

Natural and recombinant Rat Relaxin-3

Protein Length

140

NCBI Gene Symbol

Rln3

Protein Name

Q8BFS3

Gene Name URL

Rln3

Nucleotide Accession Number

NM_170667.2

Frequently Asked Questions

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