THPO ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Thrombopoietin, thrombopoietin like 1
Gene Aliases
Thpo; thrombopoietin.
Gene ID
108348162, 81811
Accession Number
NP_112395.1
Reactivity
Rat, Rattus norvegicus
Target
Thrombopoietin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology THPO ELISA Kit (Rat) (OKEH03037) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Thpo has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Thpo is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Thpo captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Sensitivity
7.86 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
35 kDa
Specificity
Natural and recombinant Rat Thrombopoietin
Protein Length
326
NCBI Gene Symbol
LOC108348162, Thpol1
Protein Name
P49745
Gene Name URL
LOC108348162, Thpol1
Nucleotide Accession Number
NM_031133.2
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