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FSTL3 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Follistatin like 3

Gene Aliases

Flrg; follistatin-like 3 (secreted glycoprotein) ; follistatin-like protein 3; follistatin-related gene protein; follistatin-related protein 3.

Gene ID

114031

Accession Number

NP_446081.1

Reactivity

Rat, Rattus norvegicus

Target

Follistatin-related protein 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FSTL3 ELISA Kit (Rat) (OKEH03033) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fstl3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Fstl3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fstl3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/ml

Sensitivity

0.088 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

27 kDa

Specificity

Natural and recombinant Rat Follistatin-related protein 3

Protein Length

256

NCBI Gene Symbol

Fstl3

Protein Name

Q99PW7

Gene Name URL

Fstl3

Nucleotide Accession Number

NM_053629.3

Frequently Asked Questions

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