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PLAUR ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Plasminogen activator, urokinase receptor

Gene Aliases

Par; plasminogen activator urokinase receptor 3; Plaur3; uPAR; U-PAR; uPAR-2; uPAR-3; urinary plasminogen activator receptor 2; urinary plasminogen activator receptor 3; urokinase plasminogen activator receptor; urokinase plasminogen activator surface receptor.

Gene ID

50692

Accession Number

NP_059046.1

Reactivity

Rat, Rattus norvegicus

Target

Urokinase plasminogen activator surface receptor

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PLAUR ELISA Kit (Rat) (OKEH03014) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Plaur has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Plaur is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Plaur captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/mL

Sensitivity

39 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

36 kDa

Specificity

Natural and recombinant Rat Urokinase plasminogen activator surface receptor

Protein Length

328

NCBI Gene Symbol

Plaur

Protein Name

P49616

Gene Name URL

Plaur

Nucleotide Accession Number

NM_017350.1

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