APP ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
Gene Name
Amyloid beta (A4) precursor protein
Gene Aliases
A; Abe; Abeta; Abpp; Adap; Ag; alzheimer disease amyloid A4 protein homolog; amyloid beta A4 protein; amyloid precursor protein; amyloid-beta A4 protein; amyloid-beta precursor protein; amyloidogenic glycoprotein; appican; bet; beta-amyloid precursor protein; betaApp; C; Cvap; E030013M08Rik; protease nexin II.
Gene ID
11820
Accession Number
NP_001185752.1
Reactivity
Mouse, Mus musculus
Target
Amyloid-beta A4 protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology APP ELISA Kit (Mouse) (OKEH03009) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for App has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for App is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample App captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 112%
Sensitivity
39 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
87 kDa
Specificity
Natural and recombinant Mouse Amyloid beta A4 protein
Protein Length
770
NCBI Gene Symbol
App
Protein Name
P12023
Gene Name URL
App
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001198823.1
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