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EBI3 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Epstein-Barr virus induced gene 3

Gene Aliases

EBI-; EBI-3; epstein-Barr virus-induced gene 3 protein homolog; IL-; IL-27; IL-27 subunit beta; IL-27B; interleukin-27 subunit beta.

Gene ID

50498

Accession Number

NP_056581.1

Reactivity

Mouse, Mus musculus

Target

Interleukin-27 subunit beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology EBI3 ELISA Kit (Mouse) (OKEH03003) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ebi3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Ebi3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ebi3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%

Sensitivity

8.7 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

25 kDa

Specificity

Natural and recombinant Mouse Interleukin-27 subunit beta

Protein Length

228

NCBI Gene Symbol

Ebi3

Protein Name

O35228

Gene Name URL

Ebi3

Nucleotide Accession Number

NM_015766.2

Frequently Asked Questions

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