What is the catalog number for NOS3 ELISA Kit (Rat)?

The catalog number for NOS3 ELISA Kit (Rat) is OKEH02990-96W.

What sizes are available for NOS3 ELISA Kit (Rat)?

NOS3 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is NOS3 ELISA Kit (Rat) in stock?

NOS3 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

NOS3 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Nitric oxide synthase 3

Gene Aliases

CNOS; constitutive NOS; EC-NOS; endothelial nitric oxide synthase 3; endothelial NOS; eNos; nitric oxide synthase 3, endothelial cell; nitric oxide synthase, endothelial; NOS type III; NOSIII.

Gene ID

24600

Accession Number

NP_068610.1

Reactivity

Rat, Rattus norvegicus

Target

Nitric oxide synthase, endothelial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NOS3 ELISA Kit (Rat) (OKEH02990) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Nos3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Nos3 and incubated. The Nos3 found in the sample or standards competes with the biotinylated Nos3 for limited binding sites on the immobilized anti-rat Nos3 antibody. Excess unbound biotinylated Nos3 and sample or standard Nos3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Nos3 captured in the well and inversely proportional to the amount of Nos3 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol