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TGFA ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Transforming growth factor alpha

Gene Aliases

Protransforming growth factor alpha; RATTGFAA; TGFAA.

Gene ID

24827

Accession Number

NP_036803.1

Reactivity

Rat, Rattus norvegicus

Target

Protransforming growth factor alpha

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TGFA ELISA Kit (Rat) (OKEH02978) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tgfa has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Tgfa is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tgfa captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

31.2-2000pg/mL

Sensitivity

15.79 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

17 kDa

Specificity

Natural and recombinant Rat Protransforming growth factor alpha

Protein Length

159

NCBI Gene Symbol

Tgfa

Protein Name

P01134

Gene Name URL

Tgfa

Nucleotide Accession Number

NM_012671.2

Frequently Asked Questions

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