TNFRSF11B ELISA Kit (Rat)
Product Specifications
Gene Name
TNF receptor superfamily member 11B
Gene Aliases
Opg; Osteoprotegerin; tumor necrosis factor receptor superfamily member 11B.
Gene ID
25341
Accession Number
NP_037002.1
Reactivity
Rat, Rattus norvegicus
Target
Tumor necrosis factor receptor superfamily member 11B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TNFRSF11B ELISA Kit (Rat) (OKEH02974) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Tnfrsf11b has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Tnfrsf11b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Tnfrsf11b captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Sensitivity
7.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
46 kDa
Specificity
Natural and recombinant Rat Tumor necrosis factor receptor superfamily member 11B
Protein Length
401
NCBI Gene Symbol
Tnfrsf11b
Protein Name
O08727
Gene Name URL
Tnfrsf11b
CAS Number
7732-18-5
Nucleotide Accession Number
NM_012870.2
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