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CXCL2 ELISA Kit (Rat)

Selected Variant

OKEH02971-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

C-X-C motif chemokine ligand 2

Gene Aliases

Chemokine (C-X-C motif) ligand 2; CINC-3; C-X-C motif chemokine 2; cytokine-induced neutrophil chemoattractant 3; macrophage inflammatory protein 2; Mip-2; MIP2; Scyb2; small inducible cytokine subfamily, member 2.

Gene ID

114105

Accession Number

NP_446099.1

Reactivity

Rat, Rattus norvegicus

Target

C-X-C motif chemokine 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CXCL2 ELISA Kit (Rat) (OKEH02971) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cxcl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

15.6-1000pg/mL

Sensitivity

7.91 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

11 kDa

Specificity

Natural and recombinant Rat C-X-C motif chemokine 2

Protein Length

100

NCBI Gene Symbol

Cxcl2

Protein Name

P30348

Gene Name URL

Cxcl2

Nucleotide Accession Number

NM_053647.1

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