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CGA ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-assay CV%:

CAS Number

7732-18-5

Gene Name

Glycoprotein hormones, alpha subunit

Gene Aliases

AG; aGSU; al; alp; alph; alphaGSU; alpha-GSU; alphaSU; anterior pituitary glycoprotein hormones common subunit alpha; CG-alpha; follicle-stimulating hormone alpha chain; follitropin alpha chain; FSHA; FSH-alpha; glycoprotein hormones alpha chain; GPH; GPHA1; GPHalpha; HCG; LHA; LSH-alpha; luteinizing hormone alpha chain; lutropin alpha chain; thyroid-stimulating hormone alpha chain; thyrotropin alpha chain; Ts; Tsha; TSH-alpha.

Gene ID

12640

Accession Number

NP_034019.1

Reactivity

Mouse, Mus musculus

Target

Glycoprotein hormones alpha chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CGA ELISA Kit (Mouse) (OKEH02958) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cga has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cga is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cga captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

3.12-200uIU/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82%

Sensitivity

1.56uIU/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

14 kDa

Specificity

Natural and recombinant Mouse Glycoprotein hormones alpha chain

Protein Length

120

NCBI Gene Symbol

Cga

Protein Name

P01216

Gene Name URL

Cga

Nucleotide Accession Number

NM_009889.2

Frequently Asked Questions

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