CGA ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Glycoprotein hormones, alpha subunit
Gene Aliases
AG; aGSU; al; alp; alph; alphaGSU; alpha-GSU; alphaSU; anterior pituitary glycoprotein hormones common subunit alpha; CG-alpha; follicle-stimulating hormone alpha chain; follitropin alpha chain; FSHA; FSH-alpha; glycoprotein hormones alpha chain; GPH; GPHA1; GPHalpha; HCG; LHA; LSH-alpha; luteinizing hormone alpha chain; lutropin alpha chain; thyroid-stimulating hormone alpha chain; thyrotropin alpha chain; Ts; Tsha; TSH-alpha.
Gene ID
12640
Accession Number
NP_034019.1
Reactivity
Mouse, Mus musculus
Target
Glycoprotein hormones alpha chain
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CGA ELISA Kit (Mouse) (OKEH02958) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cga has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cga is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cga captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
3.12-200uIU/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82%
Sensitivity
1.56uIU/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
14 kDa
Specificity
Natural and recombinant Mouse Glycoprotein hormones alpha chain
Protein Length
120
NCBI Gene Symbol
Cga
Protein Name
P01216
Gene Name URL
Cga
Nucleotide Accession Number
NM_009889.2
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog