LMNA ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-assay CV%:
CAS Number
7732-18-5
Gene Name
Lamin A/C
Gene Aliases
70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; IDC; lamin; lamin A/C-like 1; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; mandibuloacral dysplasia type A; prelamin-A/C; PRO1; renal carcinoma antigen NY-REN-32.
Gene ID
4000
Accession Number
NP_001244303.1
Reactivity
Homo sapiens, Human
Target
Prelamin-A/C
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LMNA ELISA Kit (Human) (OKEH02955) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LMNA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for LMNA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LMNA captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
40.8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
74 kDa
Specificity
Natural and recombinant Human Prelamin-A/C
Protein Length
664
NCBI Gene Symbol
LMNA
Protein Name
P02545
Gene Name URL
LMNA
Nucleotide Accession Number
NM_001257374.2
Frequently Asked Questions
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