WISP1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Cellular communication network factor 4
Gene Aliases
CCN family member 4; WISP1; WISP1 3'UTR-associated RNA 1; WISP1 overlapping transcript 1 (non-protein coding) ; WISP1c; WISP1i; WISP1-OT1; WISP1tc; WISP1-UT1; WNT1 induced secreted protein 1; WNT1 inducible signaling pathway protein 1; Wnt-1-induced secreted protein.
Gene ID
8840
Accession Number
NP_001191798.1
Reactivity
Homo sapiens, Human
Target
WNT1-inducible-signaling pathway protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology WISP1 ELISA Kit (Human) (OKEH02950) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WISP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WISP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample WISP1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
11.7 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
40 kDa
Specificity
Natural and recombinant Human WNT1-inducible-signaling pathway protein 1
Protein Length
367
NCBI Gene Symbol
CCN4
Protein Name
O95388
Gene Name URL
CCN4
Nucleotide Accession Number
NM_001204869.1
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