TNFSF13B ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
TNF superfamily member 13b
Gene Aliases
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; B-cell-activating factor; B-lymphocyte stimulator; BLYS; CD257; delta BAFF; Delta4 BAFF; dendritic cell-derived TNF-like molecule; DTL; epididymis secretory sperm binding protein; TALL1; TALL-1; THANK; TNF and ApoL-related leukocyte expressed ligand 1; TNF- and APOL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF20; TNLG7A; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4.
Gene ID
10673
Accession Number
NP_001139117.1
Reactivity
Homo sapiens, Human
Target
Tumor necrosis factor ligand superfamily member 13B
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TNFSF13B ELISA Kit (Human) (OKEH02941) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFSF13B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFSF13B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFSF13B captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
Sensitivity
0.2 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
31 kDa
Specificity
Natural and recombinant Human Tumor necrosis factor ligand superfamily member 13B
Protein Length
285
NCBI Gene Symbol
TNFSF13B
Protein Name
Q9Y275
Gene Name URL
TNFSF13B
Nucleotide Accession Number
NM_001145645.2
Frequently Asked Questions
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