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TNFSF13B ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

TNF superfamily member 13b

Gene Aliases

ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; B-cell-activating factor; B-lymphocyte stimulator; BLYS; CD257; delta BAFF; Delta4 BAFF; dendritic cell-derived TNF-like molecule; DTL; epididymis secretory sperm binding protein; TALL1; TALL-1; THANK; TNF and ApoL-related leukocyte expressed ligand 1; TNF- and APOL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF20; TNLG7A; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand 7A; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4.

Gene ID

10673

Accession Number

NP_001139117.1

Reactivity

Homo sapiens, Human

Target

Tumor necrosis factor ligand superfamily member 13B

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TNFSF13B ELISA Kit (Human) (OKEH02941) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFSF13B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFSF13B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFSF13B captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%

Sensitivity

0.2 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

31 kDa

Specificity

Natural and recombinant Human Tumor necrosis factor ligand superfamily member 13B

Protein Length

285

NCBI Gene Symbol

TNFSF13B

Protein Name

Q9Y275

Gene Name URL

TNFSF13B

Nucleotide Accession Number

NM_001145645.2

Frequently Asked Questions

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