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TNFRSF11A ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

TNF receptor superfamily member 11a

Gene Aliases

CD265; familial expansile osteolysis; FEO; LOH18CR1; loss of heterozygosity, 18, chromosomal region 1; ODFR; OFE; OPTB7; osteoclast differentiation factor receptor; OSTS; Paget disease of bone 2; PDB2; RANK; receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TRANCE receptor; TRANCER; TRANCE-R; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, NFKB activator.

Gene ID

8792

Accession Number

NP_001257878.1

Reactivity

Homo sapiens, Human

Target

Tumor necrosis factor receptor superfamily member 11A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TNFRSF11A ELISA Kit (Human) (OKEH02936) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFRSF11A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFRSF11A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFRSF11A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.067 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

66 kDa

Specificity

Natural and recombinant Human Tumor necrosis factor receptor superfamily member 11A

Protein Length

616

NCBI Gene Symbol

TNFRSF11A

Protein Name

Q9Y6Q6

Gene Name URL

TNFRSF11A

Nucleotide Accession Number

NM_001270949.1

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