What is the catalog number for TFF1 ELISA Kit (Human)?

The catalog number for TFF1 ELISA Kit (Human) is OKEH02920-96W.

What sizes are available for TFF1 ELISA Kit (Human)?

TFF1 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is TFF1 ELISA Kit (Human) in stock?

TFF1 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

TFF1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Trefoil factor 1

Gene Aliases

BCEI; breast cancer estrogen-inducible protein; breast cancer estrogen-inducible sequence; D21S21; gastrointestinal trefoil protein pS2; HP1.A; HPS2; pNR-2; polypeptide P1.A; protein pS2; pS2; trefoil factor 1.

Gene ID

7031

Accession Number

NP_003216.1

Reactivity

Homo sapiens, Human

Target

Trefoil factor 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology TFF1 ELISA Kit (Human) (OKEH02920) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human TFF1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TFF1 and incubated. The TFF1 found in the sample or standards competes with the biotinylated TFF1 for limited binding sites on the immobilized anti-human TFF1 antibody. Excess unbound biotinylated TFF1 and sample or standard TFF1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TFF1 captured in the well and inversely proportional to the amount of TFF1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol