TBX3 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
T-box transcription factor 3
Gene Aliases
Bladder cancer related protein XHL; T-box 3; T-box protein 3; T-box transcription factor TBX3; TBX3-ISO; UMS; XHL.
Gene ID
6926
Accession Number
NP_005987.3
Reactivity
Homo sapiens, Human
Target
T-box transcription factor TBX3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TBX3 ELISA Kit (Human) (OKEH02917) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TBX3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TBX3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TBX3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.13 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
79 kDa
Specificity
Natural and recombinant Human T-box transcription factor TBX3
Protein Length
743
NCBI Gene Symbol
TBX3
Protein Name
O15119
Gene Name URL
TBX3
Nucleotide Accession Number
NM_005996.3
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