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SOD1 ELISA Kit (Human)

Selected Variant

OKEH02905-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Superoxide dismutase 1

Gene Aliases

ALS; ALS1; Cu/Zn superoxide dismutase; epididymis secretory protein Li 44; HEL-S-44; homodimer; hSod1; indophenoloxidase A; IPOA; SOD; SOD, soluble; STAHP; superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; superoxide dismutase 1, soluble; superoxide dismutase, cystolic.

Gene ID

6647

Accession Number

NP_000445.1

Reactivity

Homo sapiens, Human

Target

Superoxide dismutase [Cu-Zn]

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SOD1 ELISA Kit (Human) (OKEH02905) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SOD1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SOD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SOD1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

15 kDa

Specificity

Natural and recombinant Human Superoxide dismutase [Cu-Zn]

Protein Length

154

NCBI Gene Symbol

SOD1

Protein Name

P00441

Gene Name URL

SOD1

Nucleotide Accession Number

NM_000454.4

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