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RBP4 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Retinol binding protein 4

Gene Aliases

MCOPCB10; plasma retinol-binding protein; PRBP; RBP; RDCCAS; retinol binding protein 4, plasma; retinol-binding protein 4; retinol-binding protein 4, interstitial.

Gene ID

5950

Accession Number

NP_006735.2

Reactivity

Homo sapiens, Human

Target

Retinol-binding protein 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RBP4 ELISA Kit (Human) (OKEH02885) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RBP4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RBP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RBP4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.052 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

23 kDa

Specificity

Natural and recombinant Human Retinol-binding protein 4

Protein Length

201

NCBI Gene Symbol

RBP4

Protein Name

P02753

Gene Name URL

RBP4

Nucleotide Accession Number

NM_006744.3

Frequently Asked Questions

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