What is the catalog number for MME ELISA Kit (Human)?

The catalog number for MME ELISA Kit (Human) is OKEH02832-96W.

What sizes are available for MME ELISA Kit (Human)?

MME ELISA Kit (Human) is available in the following size(s): 96 Well.

Is MME ELISA Kit (Human) in stock?

MME ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

Is this product animal-origin-free?

Animal-origin-free status, where applicable, is noted on the datasheet under formulation details.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

MME ELISA Kit (Human)

Selected Variant

OKEH02832-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Membrane metalloendopeptidase

Gene Aliases

Atriopeptidase; CALLA; CD10; CMT2T; common acute lymphocytic leukemia antigen; Enkephalinase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) ; membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; NEP; neprilysin; neprilysin-390; neprilysin-411; neutral endopeptidase 24.11; SCA43; SFE; skin fibroblast elastase.

Gene ID

4311

Accession Number

NP_000893.2

Reactivity

Homo sapiens, Human

Target

Neprilysin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MME ELISA Kit (Human) (OKEH02832) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MME has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MME is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MME captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.05 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

86 kDa

Specificity

Natural and recombinant Human Neprilysin

Protein Length

750

NCBI Gene Symbol

MME

Protein Name

P08473

Gene Name URL

MME

Nucleotide Accession Number

NM_000902.3

Price

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