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MICA ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

MHC class I polypeptide-related sequence A

Gene Aliases

HLA class I antigen; major histocompatibility complex class I chain-related protein A; MHC class I chain-related protein A; MHC class I polypeptide-related sequence A; MHC class I related chain A; MHC class I related sequence A; MIC-A; PERB11.1; stress inducible class I homolog; truncated MHC class I polypeptide-related sequence A; truncated MICA.

Gene ID

100507436

Accession Number

NP_000238.1

Reactivity

Homo sapiens, Human

Target

MHC class I polypeptide-related sequence A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MICA ELISA Kit (Human) (OKEH02830) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MICA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MICA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MICA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%

Sensitivity

33 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

43 kDa

Specificity

Natural and recombinant Human MHC class I polypeptide-related sequence A

Protein Length

383

NCBI Gene Symbol

MICA

Protein Name

Q29983

Gene Name URL

MICA

Nucleotide Accession Number

NM_000247.2

Frequently Asked Questions

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