IGF1R ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Insulin like growth factor 1 receptor
Gene Aliases
CD221; IGF-I receptor; IGFIR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; soluble IGF1R variant 1; soluble IGF1R variant 2.
Gene ID
3480
Accession Number
NP_000866.1
Reactivity
Homo sapiens, Human
Target
Insulin-like growth factor 1 receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology IGF1R ELISA Kit (Human) (OKEH02784) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IGF1R has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IGF1R is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IGF1R captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%
Sensitivity
0.098 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
155 kDa
Specificity
Natural and recombinant Human Insulin-like growth factor 1 receptor
Protein Length
1367
NCBI Gene Symbol
IGF1R
Protein Name
P08069
Gene Name URL
IGF1R
Nucleotide Accession Number
NM_000875.4
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