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HSPA4 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Heat shock protein family A (Hsp70) member 4

Gene Aliases

APG-2; epididymis secretory sperm binding protein Li 5a; heat shock 70 kDa protein 4; heat shock 70kD protein 4; heat shock 70-related protein APG-2; heat shock protein, 110 kDa; HEL-S-5a; HS24/P52; hsp70; hsp70 RY; hsp70RY; HSPH2; RY.

Gene ID

3308

Accession Number

NP_002145.3

Reactivity

Homo sapiens, Human

Target

Heat shock 70 kDa protein 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology HSPA4 ELISA Kit (Human) (OKEH02781) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSPA4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSPA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSPA4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

0.12 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

94 kDa

Specificity

Natural and recombinant Human Heat shock 70 kDa protein 4

Protein Length

840

NCBI Gene Symbol

HSPA4

Protein Name

P34932

Gene Name URL

HSPA4

Nucleotide Accession Number

NM_002154.3

Frequently Asked Questions

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