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GLB1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Galactosidase beta 1

Gene Aliases

Acid beta-galactosidase; beta-galactosidase; EBP; elastin binding protein; Elastin receptor 1; elastin receptor 1, 67kDa; ELNR1; lactase; MPS4B.

Gene ID

2720

Accession Number

NP_000395.2

Reactivity

Homo sapiens, Human

Target

Beta-galactosidase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology GLB1 ELISA Kit (Human) (OKEH02757) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GLB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GLB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample GLB1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

12.5-800pg/mL

Sensitivity

9.8 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

76 kDa

Specificity

Natural and recombinant Human Beta-galactosidase

Protein Length

677

NCBI Gene Symbol

GLB1

Protein Name

P16278

Gene Name URL

GLB1

Nucleotide Accession Number

NM_000404.3

Frequently Asked Questions

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