FGF7 ELISA Kit (Pig)
Product Specifications
Gene Name
Fibroblast growth factor 7
Gene Aliases
Heparin-binding growth factor 7; Keratinocyte growth factor.
Gene ID
397281
Accession Number
XP_013848480.1
Reactivity
Porcine, Sus scrofa
Target
Fibroblast growth factor 7
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FGF7 ELISA Kit (Pig) (OKEH02746) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGF7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FGF7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGF7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Sensitivity
8 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
22 kDa
Specificity
Natural and recombinant Pig Fibroblast growth factor 7
Protein Length
194
NCBI Gene Symbol
FGF7
Protein Name
Q9N198
Gene Name URL
FGF7
CAS Number
7732-18-5
Nucleotide Accession Number
XM_013993026.1
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