Fgf23 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Fibroblast growth factor 23
Gene Aliases
FGF-23; Fgf8b; fibroblast growth factor 23; fibroblast growth factor 8b.
Gene ID
170583
Accession Number
NP_570110.1
Reactivity
Rat, Rattus norvegicus
Target
Fibroblast growth factor 23
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Fgf23 ELISA Kit (Rat) (OKEH02744) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Fgf23 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Fgf23 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fgf23 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
8.02 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
28 kDa
Specificity
Natural and recombinant Rat Fibroblast growth factor 23
Protein Length
251
NCBI Gene Symbol
Fgf23
Protein Name
Q8VI82
Gene Name URL
Fgf23
Nucleotide Accession Number
NM_130754.1
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