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FCN2 ELISA Kit (Human)

Selected Variant

OKEH02741-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Ficolin 2

Gene Aliases

37 kDa elastin-binding protein; collagen/fibrinogen domain-containing protein 2; EBP-37; FCNL; ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin) ; ficolin B; ficolin-2; Ficolin-B; ficolin-beta; Hucolin; L-ficolin; P35; serum lectin p35.

Gene ID

2220

Accession Number

NP_004099.2

Reactivity

Homo sapiens, Human

Target

Ficolin-2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FCN2 ELISA Kit (Human) (OKEH02741) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FCN2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FCN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FCN2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%

Sensitivity

20 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

34 kDa

Specificity

Natural and recombinant Human Ficolin-2

Protein Length

313

NCBI Gene Symbol

FCN2

Protein Name

Q15485

Gene Name URL

FCN2

Nucleotide Accession Number

NM_004108.2

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