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DPP4 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Dipeptidyl peptidase 4

Gene Aliases

ADABP; ADCP2; ADCP-2; adenosine deaminase complexing protein 2; CD26; dipeptidyl peptidase 4; dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2) ; DPP IV; DPPIV; Gly-Pro naphthylamidase; Post-proline dipeptidyl aminopeptidase IV; T-cell activation antigen CD26; TP103; Xaa-Pro-dipeptidylaminopeptidase.

Gene ID

1803

Accession Number

NP_001926.2

Reactivity

Homo sapiens, Human

Target

Dipeptidyl peptidase 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DPP4 ELISA Kit (Human) (OKEH02728) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DPP4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for DPP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DPP4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

62.5-4000ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%

Sensitivity

30.5 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

88 kDa

Specificity

Natural and recombinant Human Dipeptidyl peptidase 4

Protein Length

766

NCBI Gene Symbol

DPP4

Protein Name

P27487

Gene Name URL

DPP4

Nucleotide Accession Number

NM_001935.3

Frequently Asked Questions

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