CHI3L1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Chitinase 3 like 1
Gene Aliases
39 kDa synovial protein; ASRT7; cartilage glycoprotein 39; CGP-39; chitinase 3-like 1 (cartilage glycoprotein-39) ; chitinase-3-like protein 1; GP39; GP-39; hCGP-39; HC-gp39; HCGP-3P; YK-40; YKL40; YKL-40; YYL-40.
Gene ID
1116
Accession Number
NP_001267.2
Reactivity
Homo sapiens, Human
Target
Chitinase-3-like protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CHI3L1 ELISA Kit (Human) (OKEH02691) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human CHI3L1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CHI3L1 and incubated. The CHI3L1 found in the sample or standards competes with the biotinylated CHI3L1 for limited binding sites on the immobilized anti-Human CHI3L1 antibody. Excess unbound biotinylated CHI3L1 and sample or standard CHI3L1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CHI3L1 captured in the well and inversely proportional to the amount of CHI3L1 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
12 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
43 kDa
Specificity
Natural and recombinant Human Chitinase-3-like protein 1
Protein Length
383
NCBI Gene Symbol
CHI3L1
Protein Name
P36222
Gene Name URL
CHI3L1
Nucleotide Accession Number
NM_001276.2
Frequently Asked Questions
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