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CHI3L1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Chitinase 3 like 1

Gene Aliases

39 kDa synovial protein; ASRT7; cartilage glycoprotein 39; CGP-39; chitinase 3-like 1 (cartilage glycoprotein-39) ; chitinase-3-like protein 1; GP39; GP-39; hCGP-39; HC-gp39; HCGP-3P; YK-40; YKL40; YKL-40; YYL-40.

Gene ID

1116

Accession Number

NP_001267.2

Reactivity

Homo sapiens, Human

Target

Chitinase-3-like protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CHI3L1 ELISA Kit (Human) (OKEH02691) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human CHI3L1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CHI3L1 and incubated. The CHI3L1 found in the sample or standards competes with the biotinylated CHI3L1 for limited binding sites on the immobilized anti-Human CHI3L1 antibody. Excess unbound biotinylated CHI3L1 and sample or standard CHI3L1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CHI3L1 captured in the well and inversely proportional to the amount of CHI3L1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%

Sensitivity

12 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

43 kDa

Specificity

Natural and recombinant Human Chitinase-3-like protein 1

Protein Length

383

NCBI Gene Symbol

CHI3L1

Protein Name

P36222

Gene Name URL

CHI3L1

Nucleotide Accession Number

NM_001276.2

Frequently Asked Questions

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