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CEBPE ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

CCAAT enhancer binding protein epsilon

Gene Aliases

C/EBP epsilon; C/EBP-epsilon; CCAAT/enhancer binding protein (C/EBP), epsilon; CCAAT/enhancer-binding protein epsilon; CRP1.

Gene ID

1053

Accession Number

NP_001796.2

Reactivity

Homo sapiens, Human

Target

CCAAT/enhancer-binding protein epsilon

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CEBPE ELISA Kit (Human) (OKEH02688) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CEBPE has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CEBPE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CEBPE captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.23 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

31 kDa

Specificity

Natural and recombinant Human CCAAT/enhancer-binding protein epsilon

Protein Length

281

NCBI Gene Symbol

CEBPE

Protein Name

Q15744

Gene Name URL

CEBPE

Nucleotide Accession Number

NM_001805.3

Frequently Asked Questions

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