What is the catalog number for Casp3 ELISA Kit (Rat)?

The catalog number for Casp3 ELISA Kit (Rat) is OKEH02671-96W.

What sizes are available for Casp3 ELISA Kit (Rat)?

Casp3 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is Casp3 ELISA Kit (Rat) in stock?

Casp3 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Casp3 ELISA Kit (Rat)

Selected Variant

OKEH02671-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Caspase 3

Gene Aliases

Apopain; CASP-3; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease) ; caspase-3; CPP-32; CPP32beta; CPP32-beta; cysteine protease CPP32; cysteine protease p32-beta; IRP; Lice; protein Yama; SCA-1; SREBP cleavage activity 1; Yama.

Gene ID

25402

Accession Number

NP_037054.1

Reactivity

Rat, Rattus norvegicus

Target

Caspase-3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Casp3 ELISA Kit (Rat) (OKEH02671) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Casp3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Casp3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Casp3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%

Sensitivity

0.156 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

31 kDa

Notes

Formerly GWB-4Z93FL

Specificity

Natural and recombinant Rat Caspase-3

Protein Length

277

NCBI Gene Symbol

Casp3

Protein Name

P55213

Gene Name URL

Casp3

Nucleotide Accession Number

NM_012922.2

Price

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