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Calm1 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Calmodulin 1, calmodulin 2, calmodulin 3

Gene Aliases

Calm; Calmodulin 1 (phosphorylase kinase, delta) ; calmodulin 2 (phosphorylase kinase, delta) ; calmodulin I; Calmodulin III; calmodulin-1; calmodulin-2; calmodulin-3; caM; Cam1; Cam2; Cam3; Camb; Camc; CaMI; CaMII; CaMIII; RNCAMIII.

Gene ID

24242, 24244, 50663

Accession Number

NP_114175.1

Reactivity

Rat, Rattus norvegicus

Target

Calmodulin-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology Calm1 ELISA Kit (Rat) (OKEH02668) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Calm1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Calm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Calm1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

1.56-100ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%

Sensitivity

0.78 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

17 kDa

Specificity

Natural and recombinant Rat Calmodulin

Protein Length

149

NCBI Gene Symbol

Calm1, Calm2, Calm3

Protein Name

P0DP29

Gene Name URL

Calm1, Calm2, Calm3

Nucleotide Accession Number

NM_031969.2

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