Calm1 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Calmodulin 1, calmodulin 2, calmodulin 3
Gene Aliases
Calm; Calmodulin 1 (phosphorylase kinase, delta) ; calmodulin 2 (phosphorylase kinase, delta) ; calmodulin I; Calmodulin III; calmodulin-1; calmodulin-2; calmodulin-3; caM; Cam1; Cam2; Cam3; Camb; Camc; CaMI; CaMII; CaMIII; RNCAMIII.
Gene ID
24242, 24244, 50663
Accession Number
NP_114175.1
Reactivity
Rat, Rattus norvegicus
Target
Calmodulin-1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology Calm1 ELISA Kit (Rat) (OKEH02668) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Calm1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Calm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Calm1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Sensitivity
0.78 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
17 kDa
Specificity
Natural and recombinant Rat Calmodulin
Protein Length
149
NCBI Gene Symbol
Calm1, Calm2, Calm3
Protein Name
P0DP29
Gene Name URL
Calm1, Calm2, Calm3
Nucleotide Accession Number
NM_031969.2
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