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CALB2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Calbindin 2

Gene Aliases

29 kDa calbindin; CAB29; CAL2; calbindin 2, (29kD, calretinin) ; calbindin D29K; calretinin; CR; testicular secretory protein Li 8.

Gene ID

794

Accession Number

NP_001731.2

Reactivity

Homo sapiens, Human

Target

Calretinin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CALB2 ELISA Kit (Human) (OKEH02666) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CALB2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CALB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CALB2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

31 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

32 kDa

Specificity

Natural and recombinant Human Calretinin

Protein Length

271

NCBI Gene Symbol

CALB2

Protein Name

P22676

Gene Name URL

CALB2

Nucleotide Accession Number

NM_001740.4

Frequently Asked Questions

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