AQP3 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Aquaporin 3 (Gill blood group)
Gene Aliases
AQP-3; aquaglyceroporin-3; aquaporin 3 (GIL blood group) ; aquaporin-3; GIL.
Gene ID
360
Accession Number
NP_004916.1
Reactivity
Homo sapiens, Human
Target
Aquaporin-3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology AQP3 ELISA Kit (Human) (OKEH02650) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human AQP3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AQP3 and incubated. The AQP3 found in the sample or standards competes with the biotinylated AQP3 for limited binding sites on the immobilized anti-Human AQP3 antibody. Excess unbound biotinylated AQP3 and sample or standard AQP3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AQP3 captured in the well and inversely proportional to the amount of AQP3 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.072 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
32 kDa
Specificity
Natural and recombinant Human Aquaporin-3
Protein Length
292
NCBI Gene Symbol
AQP3
Protein Name
Q92482
Gene Name URL
AQP3
Nucleotide Accession Number
NM_004925.4
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog