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AQP3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Aquaporin 3 (Gill blood group)

Gene Aliases

AQP-3; aquaglyceroporin-3; aquaporin 3 (GIL blood group) ; aquaporin-3; GIL.

Gene ID

360

Accession Number

NP_004916.1

Reactivity

Homo sapiens, Human

Target

Aquaporin-3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology AQP3 ELISA Kit (Human) (OKEH02650) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human AQP3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AQP3 and incubated. The AQP3 found in the sample or standards competes with the biotinylated AQP3 for limited binding sites on the immobilized anti-Human AQP3 antibody. Excess unbound biotinylated AQP3 and sample or standard AQP3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AQP3 captured in the well and inversely proportional to the amount of AQP3 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.072 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

32 kDa

Specificity

Natural and recombinant Human Aquaporin-3

Protein Length

292

NCBI Gene Symbol

AQP3

Protein Name

Q92482

Gene Name URL

AQP3

Nucleotide Accession Number

NM_004925.4

Frequently Asked Questions

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