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APOB ELISA Kit (Human)

Selected Variant

OKEH02645-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Apolipoprotein B

Gene Aliases

ApoB-100; apoB-48; apolipoprotein B (including Ag (x) antigen) ; apolipoprotein B-100; apolipoprotein B48; FCHL2; FLDB; LDLCQ4.

Gene ID

338

Accession Number

NP_000375.2

Reactivity

Homo sapiens, Human

Target

Apolipoprotein B-100

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology APOB ELISA Kit (Human) (OKEH02645) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for APOB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for APOB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample APOB captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Sensitivity

20 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

516 kDa

Specificity

Natural and recombinant Human Apolipoprotein B-100

Protein Length

4563

NCBI Gene Symbol

APOB

Protein Name

P04114

Gene Name URL

APOB

Nucleotide Accession Number

NM_000384.2

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