ANGPTL3 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Angiopoietin like 3
Gene Aliases
ANG-5; angiopoietin 5; Angiopoietin-5; Angiopoietin-like protein 3; angiopoietin-related protein 3; ANGPT5; ANL3; FHBL2.
Gene ID
27329
Accession Number
NP_055310.1
Reactivity
Homo sapiens, Human
Target
Angiopoietin-related protein 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ANGPTL3 ELISA Kit (Human) (OKEH02641) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANGPTL3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ANGPTL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ANGPTL3 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.235-15ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%
Sensitivity
0.1 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
54 kDa
Specificity
Natural and recombinant Human Angiopoietin-related protein 3
Protein Length
460
NCBI Gene Symbol
ANGPTL3
Protein Name
Q9Y5C1
Gene Name URL
ANGPTL3
Nucleotide Accession Number
NM_014495.3
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS7150574 Inquire
Rabbit anti-Saccharomyces cerevisiae (strain 204508/S288c)(Baker's yeast) YDR306C Polyclonal Antibody
Ask
View Details 