ADAMTS4 ELISA Kit (Human)
Product Specifications
Gene Name
ADAM metallopeptidase with thrombospondin type 1 motif 4
Gene Aliases
A disintegrin and metalloproteinase with thrombospondin motifs 4; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4; ADAM-TS 4; ADAMTS-2; ADAMTS-4; ADAM-TS4; ADMP-1; aggrecanase-1; epididymis secretory sperm binding protein.
Gene ID
9507
Accession Number
NP_001307265.1
Reactivity
Homo sapiens, Human
Target
A disintegrin and metalloproteinase with thrombospondin motifs 4
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ADAMTS4 ELISA Kit (Human) (OKEH02632) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAMTS4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ADAMTS4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAMTS4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
0.092 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
90 kDa
Specificity
Natural and recombinant Human A disintegrin and metalloproteinase with thrombospondin motifs 4
Protein Length
837
NCBI Gene Symbol
ADAMTS4
Protein Name
O75173
Gene Name URL
ADAMTS4
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001320336.1
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