ADAM12 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
ADAM metallopeptidase domain 12
Gene Aliases
ADAM12-OT1; CAR10; disintegrin and metalloproteinase domain-containing protein 12; MCMP; MCMPMltna; meltrin-alpha; metalloprotease-disintegrin 12 transmembrane; MLTN; MLTNA.
Gene ID
8038
Accession Number
NP_001275902.1
Reactivity
Homo sapiens, Human
Target
Disintegrin and metalloproteinase domain-containing protein 12
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ADAM12 ELISA Kit (Human) (OKEH02628) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAM12 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ADAM12 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ADAM12 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
100 kDa
Specificity
Natural and recombinant Human Disintegrin and metalloproteinase domain-containing protein 12
Protein Length
909
NCBI Gene Symbol
ADAM12
Protein Name
O43184
Gene Name URL
ADAM12
Nucleotide Accession Number
NM_001288973.1
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