ABP1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Amine oxidase copper containing 1
Gene Aliases
ABP; ABP1; amiloride binding protein 1 (amine oxidase (copper-containing) ) ; amiloride-binding protein 1; amiloride-sensitive amine oxidase; amiloride-sensitive amine oxidase [copper-containing]; amine oxidase copper domain-containing protein 1; DAO; DAO1; diamine oxidase; histaminase; KAO; kidney amine oxidase.
Gene ID
26
Reactivity
Homo sapiens, Human
Target
Amiloride-sensitive amine oxidase [copper-containing]
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ABP1 ELISA Kit (Human) (OKEH02625) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ABP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ABP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ABP1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
Sensitivity
0.56 ng/mL
Reconstitution
Store as indicated in product manual.
Specificity
Natural and recombinant Human Amiloride-sensitive amine oxidase [copper-containing]
NCBI Gene Symbol
AOC1
Protein Name
P19801
Gene Name URL
AOC1
Frequently Asked Questions
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