Biopterin ELISA Kit

Biopterin is a coenzyme that is produced within the body. It is an oxidized degradation product of tetrahydrobiopterin. Defects in biopterin synthesis or regeneration can cause a form of hyperphenylalaninemia (a disease with symptoms similar to PKU) . Biopterin is synthesized in several parts of the body, including the pineal gland. Biopterin deficiency has been associated with a variety of disorders, including dystonia and rabies.

Aviva Systems Biology Biopterin ELISA Kit (OKEH02614) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Biopterin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Biopterin and incubated. The Biopterin found in the sample or standards competes with the biotinylated Biopterin for limited binding sites on the immobilized anti-Biopterin antibody. Excess unbound biotinylated Biopterin and sample or standard Biopterin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Biopterin captured in the well and inversely proportional to the amount of Biopterin which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol