What is the catalog number for Lipoarabinomannan ELISA Kit?

The catalog number for Lipoarabinomannan ELISA Kit is OKEH02596-96W.

What sizes are available for Lipoarabinomannan ELISA Kit?

Lipoarabinomannan ELISA Kit is available in the following size(s): 96 Well.

Is Lipoarabinomannan ELISA Kit in stock?

Lipoarabinomannan ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

Lipoarabinomannan ELISA Kit

Product Specifications

CAS Number

7732-18-5

Gene Aliases

LAM, Lipoarabinomannan, LAM

Reactivity

All Species

Target

Lipoarabinomannan is synthesized when a mannopyranosyl adds to a phosphoinositol to produce PIMs and lipomannan (LM) . PIM and LM are then glycosylated with arabinan to form LAM. Lipoarabinomannan, also called LAM, is a glycolipid, and a virulence factor associated with Mycobacterium tuberculosis, the bacteria responsible for tuberculosis. Its primary function is to inactivate macrophages and scavenge oxidative radicals.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology Lipoarabinomannan ELISA Kit (OKEH02596) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Lipoarabinomannan antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Lipoarabinomannan and incubated. The Lipoarabinomannan found in the sample or standards competes with the biotinylated Lipoarabinomannan for limited binding sites on the immobilized anti-Lipoarabinomannan antibody. Excess unbound biotinylated Lipoarabinomannan and sample or standard Lipoarabinomannan is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Lipoarabinomannan captured in the well and inversely proportional to the amount of Lipoarabinomannan which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol