Vanillylmandelic acid ELISA Kit

Vanillyl mandelic acid (VMA) is an end-stage metabolite of the catecholamines epinephrine and norepinephrine. It is produced via intermediary metabolites. VMA is found in the urine, along with other catecholamine metabolites, including homovanillic acid (HVA), metanephrine and normetanephrine. In timed urine tests the quantity excreted (usually per 24 hours) is assessed, along with creatinine clearance, and the quantity of cortisols, catecholamines, and metanephrines excreted.

Aviva Systems Biology Vanillylmandelic acid ELISA Kit (OKEH02588) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Vanillylmandelic acid antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Vanillylmandelic acid and incubated. The Vanillylmandelic acid found in the sample or standards competes with the biotinylated Vanillylmandelic acid for limited binding sites on the immobilized anti-Vanillylmandelic acid antibody. Excess unbound biotinylated Vanillylmandelic acid and sample or standard Vanillylmandelic acid is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Vanillylmandelic acid captured in the well and inversely proportional to the amount of Vanillylmandelic acid which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol